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JAK3 antibody

Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020
Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020
Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020
Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020
Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020
Anti-JAK3 antibody used in Western Blot (WB). GTX34020
Anti-JAK3 antibody used in Western Blot (WB). GTX34020

Cat. No. GTX34020

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all JAK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:100-1:300
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthesized peptide derived from human JAK3 around the non-phosphorylation site of Y785 at 720-800 aa.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

JAK3,JAK3_HUMAN,JAKL,Janus kinase 3,LJAK

Cellular Localization

Endomembrane system,Cytoplasm

Background

The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020

GTX34020 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX34020 JAK3 antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020

GTX34020 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34020 JAK3 antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020

GTX34020 IHC-P Image

IHC-P analysis of human colon tissue using GTX34020 JAK3 antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020

GTX34020 IHC-P Image

IHC-P analysis of human liver tissue using GTX34020 JAK3 antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-JAK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX34020

GTX34020 IHC-P Image

IHC-P analysis of human stomach tissue using GTX34020 JAK3 antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-JAK3 antibody used in Western Blot (WB). GTX34020

GTX34020 WB Image

WB analysis of Jurkat whole cell lysate using GTX34020 JAK3 antibody. The lane on the right was treated with the antigen-specific peptide.

Anti-JAK3 antibody used in Western Blot (WB). GTX34020

GTX34020 WB Image

Western Blot (WB) analysis of 293T cells using JAK3 Polyclonal Antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399