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JAM-A antibody [SC60-07]

Anti-JAM-A antibody [SC60-07] used in Western Blot (WB). GTX01112
Anti-JAM-A antibody [SC60-07] used in IHC (Paraffin sections) (IHC-P). GTX01112

Cat No. GTX01112

Host

Rabbit

Clonality

Monoclonal

Clone Name

SC60-07

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2885214

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F11 receptor , CD321 , F11R , JAM , JAM1 , JAMA , JCAM , KAT , PAM1 , JAM-A , Junctional Adhesion Molecule 1

Cellular Localization

Cell junction,Tight junction,Cell membrane

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JAM-A antibody [SC60-07] used in Western Blot (WB). GTX01112

GTX01112 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with JAM-A antibody [SC60-07] (GTX01112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. and the observed expression level are based on the publication: PMID: 22647687 and PMID: 18381425.

Anti-JAM-A antibody [SC60-07] used in IHC (Paraffin sections) (IHC-P). GTX01112

GTX01112 IHC-P Image

IHC-P analysis of mouse liver tissue section using GTX01112 JAM-A antibody [SC60-07].

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369