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JAM-B antibody

Anti-JAM-B antibody used in IHC (Paraffin sections) (IHC-P). GTX104507
Anti-JAM-B antibody used in IHC (Paraffin sections) (IHC-P). GTX104507
Anti-JAM-B antibody used in Western Blot (WB). GTX104507

Cat. No. GTX104507

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all JAM-B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human JAM-B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950606

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

junctional adhesion molecule 2 , C21orf43 , CD322 , JAM-B , JAMB , PRO245 , VE-JAM , VEJAM

Cellular Localization

Cell junction , tight junction , Cell membrane; Single-pass type I membrane protein

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-JAM-B antibody used in IHC (Paraffin sections) (IHC-P). GTX104507

GTX104507 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using JAM-B(GTX104507) antibody at 1:200 dilution.(Image courtesy of Sergei Grivennikov Ph.D (Laboratory of Dr. Michael Karin, UCSD.)
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-JAM-B antibody used in IHC (Paraffin sections) (IHC-P). GTX104507

GTX104507 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using JAM-B(GTX104507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-JAM-B antibody used in Western Blot (WB). GTX104507

GTX104507 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with JAM-B antibody (GTX104507) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Grivennikov SI et al. Nature 2012; 491 (7423) : 254-8 Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169