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JARID2 antibody

Anti-JARID2 antibody used in Western Blot (WB). GTX129020
Anti-JARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129020
Anti-JARID2 antibody used in Western Blot (WB). GTX129020

Cat No. GTX129020

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

human JARID2-transfected 293T , fetal mouse forebrain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human JARID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

jumonji and AT-rich interaction domain containing 2 , JMJ

Cellular Localization

Nucleus

Background

This gene is an ortholog of the mouse jumonji gene, which encodes a nuclear protein essential for mouse embryogenesis, including neural tube formation. Overexpression of mouse jumonji negatively regulates cell proliferation. The jumonji proteins contain a DNA-binding domain, called an AT-rich interaction domain (ARID), and share regions of similarity with human retinoblastoma-binding protein-2 and the human SMCX protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-JARID2 antibody used in Western Blot (WB). GTX129020

GTX129020 WB Image

JARID2 antibody detects JARID2 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human JARID2-transfected 293T cells
5 % SDS-PAGE
JARID2 antibody (GTX129020) dilution: 1:20000

Anti-JARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129020

GTX129020 ICC/IF Image

JARID2 antibody detects JARID2 protein at nucleus by immunofluorescent analysis.
Sample: JARID2-transfected (right) or untransfected (left) HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: JARID2 protein stained by JARID2 antibody (GTX120920) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-JARID2 antibody used in Western Blot (WB). GTX129020

GTX129020 WB Image

JARID2 antibody detects JARID2 protein by western blot analysis.
A. 50 μg fetal mouse forebrain lysate/extract
5 % SDS-PAGE
JARID2 antibody (GTX129020) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169