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JIK antibody [N2C2], Internal

Anti-JIK antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102894
Anti-JIK antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102894
Anti-JIK antibody [N2C2], Internal used in Western Blot (WB). GTX102894

Cat No. GTX102894

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human JIK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TAO kinase 3 , DPK , JIK , MAP3K18 , hKFC-A

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein

Background

Inhibits the basal activity of Jun kinase. Negatively regulated by epidermal growth factor (EGF). When overexpressed, may activate ERK1/ERK2 and JNK/SAPK.

Database

DATA IMAGES
Anti-JIK antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102894

GTX102894 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using JIK(GTX102894) antibody at 1:500 dilution.

Anti-JIK antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102894

GTX102894 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using JIK(GTX102894) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-JIK antibody [N2C2], Internal used in Western Blot (WB). GTX102894

GTX102894 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
7.5% SDS PAGE
GTX102894 diluted at 1:500

REFERENCE
There are currently no references for JIK antibody [N2C2], Internal (GTX102894). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for JIK antibody [N2C2], Internal (GTX102894). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169