GeneTex
United States (US)

JIP1 antibody

GTX100604_WB_19011506_173.jpg
GTX100604_IHC_19011506_318.jpg
GTX100604_39974_20150327_IHC_M_19011506_925.jpg

Cat No. GTX100604

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human JIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IB1 antibody, JIP-1 antibody, JIP1 antibody, PRKM8IP antibody, MAPK8IP1 antibody, PRKM8 interacting protein antibody, JNK-interacting protein 1 antibody, IB-1 antibody, C-Jun-amino-terminal kinase-interacting protein 1 antibody, JNK MAP kinase scaffold protein 1 antibody, islet-brain 1 antibody, mitogen-activated protein kinase 8 interacting protein 1 antibody

Cellular Localization

Cytoplasm (By similarity) , Cytoplasm , perinuclear region (By similarity) , Nucleus (By similarity)

Background

The protein encoded by this gene is a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100604_WB_19011506_173.jpg

GTX100604 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100604 diluted at 1:1000

GTX100604_IHC_19011506_318.jpg

GTX100604 IHC-P Image

Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using MAPK8IP1(GTX100604) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100604_39974_20150327_IHC_M_19011506_925.jpg

GTX100604 IHC-P Image

JIP1 antibody detects JIP1 protein at cytoplasm on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
JIP1 antibody (GTX100604) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169