APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human JIP2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950880
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mitogen-activated protein kinase 8 interacting protein 2 , IB-2 , IB2 , JIP2 , PRKM8IPL
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is closely related to MAPK8IP1/IB1/JIP-1, a scaffold protein that is involved in the c-Jun amino-terminal kinase signaling pathway. This protein is expressed in brain and pancreatic cells. It has been shown to interact with, and regulate the activity of MAPK8/JNK1, and MAP2K7/MKK7 kinases. This protein thus is thought to function as a regulator of signal transduction by protein kinase cascade in brain and pancreatic beta-cells. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX111162 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using JIP2(GTX111162) antibody at 1:200 dilution.
|
|
GTX111162 WB Image
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX111162 diluted at 1:1000
|
|
GTX111162 WB Image
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX111162 diluted at 1:1000
|
REFERENCE
There are currently no references for JIP2 antibody [C1C3] (GTX111162). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for JIP2 antibody [C1C3] (GTX111162). Be the first to share your experience with this product.