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JIP2 antibody [C1C3]

Anti-JIP2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111162
Anti-JIP2 antibody [C1C3] used in Western Blot (WB). GTX111162
Anti-JIP2 antibody [C1C3] used in Western Blot (WB). GTX111162

Cat No. GTX111162

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all JIP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human JIP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950880

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 8 interacting protein 2 , IB-2 , IB2 , JIP2 , PRKM8IPL

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is closely related to MAPK8IP1/IB1/JIP-1, a scaffold protein that is involved in the c-Jun amino-terminal kinase signaling pathway. This protein is expressed in brain and pancreatic cells. It has been shown to interact with, and regulate the activity of MAPK8/JNK1, and MAP2K7/MKK7 kinases. This protein thus is thought to function as a regulator of signal transduction by protein kinase cascade in brain and pancreatic beta-cells. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-JIP2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111162

GTX111162 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using JIP2(GTX111162) antibody at 1:200 dilution.

Anti-JIP2 antibody [C1C3] used in Western Blot (WB). GTX111162

GTX111162 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111162 diluted at 1:1000
Anti-JIP2 antibody [C1C3] used in Western Blot (WB). GTX111162

GTX111162 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111162 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169