APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 4-6μg/ml |
|
Note :
IHC-P
Mouse Brain shows axonal staining in various regions.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX24545-PEP).
Product Note
This antibody is expected to recognise both reported Human isoforms of this protein (as represented by NP_055948.2 and NP_001035529.1).
Predict Reactivity
Human, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence MEIQMDEGGGVV-C, from the N Terminus of the protein sequence according to NP_055948.2; NP_001035529.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 8 interacting protein 3 , JIP-3 , JIP3 , JSAP1 , SYD2 , syd
Cellular Localization
Cytoplasm , Golgi apparatus , Cytoplasmic vesicle , Cell projection, dendrite
Background
The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX24545 IHC-P Image
IHC-P analysis of mouse brain using GTX24545 JIP3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
REFERENCE
There are currently no references for JIP3 antibody, N-term (GTX24545). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for JIP3 antibody, N-term (GTX24545). Be the first to share your experience with this product.
