JMJD3 antibody [C2], C-term

Name: JMJD3 antibody [C2], C-term
Brand: GeneTex
SKU: GTX124222
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-JMJD3 antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124222

Anti-JMJD3 antibody [C2], C-term used in Western Blot (WB). GTX124222

Cat. No. GTX124222

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all JMJD3 products

View product citations for antibody GTX124222 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
Note : WB Recommend blocking with 1% skim milk.

Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

A375

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human JMJD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 6B , JMJD3

Database

Gene ID: 23135 KDM6B
UniProt: O15054 KDM6B

Research Area

DATA IMAGES

GTX124222 IHC-P Image

JMJD3 antibody [C2], C-term detects JMJD3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
JMJD3 stained by JMJD3 antibody [C2], C-term (GTX124222) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124222 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with JMJD3 antibody [C2], C-term (GTX124222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX124222 IHC-P Image

JMJD3 antibody [C2], C-term detects JMJD3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
JMJD3 stained by JMJD3 antibody [C2], C-term (GTX124222) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.

Tang QY et al. Stem Cell Reports 2020; 15 (2) : 439-453 UTX Regulates Human Neural Differentiation and Dendritic Morphology by Resolving Bivalent Promoters.

Application : WB

Reactivity : Human

Nakayama H et al. Anticancer Res 2020; 40 (4) : 2133-2139 EZH2 and MMSET Were Identified as Potentially Useful Therapeutic Targets in Metaplastic Breast Carcinoma.

Application : IHC-P

Reactivity : Human

Yang L et al. Oncogenesis 2019; 8 (1) : 3 Histone demethylase KDM6B has an anti-tumorigenic function in neuroblastoma by promoting differentiation.

Application : WB

Reactivity : Human

Perrigue PM et al. Mol Cancer Res 2015; 13 (4) : 636-50 The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines.

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REVIEW