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JMJD3 antibody [C2], C-term

Anti-JMJD3 antibody [C2], C-term used in Western Blot (WB). GTX124222
Anti-JMJD3 antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124222
Anti-JMJD3 antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124222

Cat No. GTX124222

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

293T , A375

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human JMJD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine demethylase 6B , JMJD3

Database

Research Area

DATA IMAGES

Anti-JMJD3 antibody [C2], C-term used in Western Blot (WB). GTX124222

GTX124222 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with JMJD3 antibody [C2], C-term (GTX124222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-JMJD3 antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124222

GTX124222 IHC-P Image

JMJD3 antibody [C2], C-term detects JMJD3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
JMJD3 stained by JMJD3 antibody [C2], C-term (GTX124222) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-JMJD3 antibody [C2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124222

GTX124222 IHC-P Image

JMJD3 antibody [C2], C-term detects JMJD3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
JMJD3 stained by JMJD3 antibody [C2], C-term (GTX124222) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

JMJD3 antibody [C2], C-term Cat No. GTX124222

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169