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JMJD3 antibody

Orthogonal
Anti-JMJD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31466
Anti-JMJD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31466
Anti-JMJD3 antibody used in Western Blot (WB). GTX31466

Cat. No. GTX31466

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all JMJD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/mL
ICC/IF 2.5 μg/mL
IHC-Fr Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , HeLa Cell Lysate

Product Note

This antibody is specific for JMJD3 and will not recognize other JMJD proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

JMJD3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human JMJD3.The immunogen is located within amino acids 1370 - 1420 of JMJD3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 6B , JMJD3

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-JMJD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31466

GTX31466 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31466 JMJD3 antibody.
Working concentration : 2.5 μg/ml

Anti-JMJD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31466

GTX31466 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31466 JMJD3 antibody.
Working concentration : 20 μg/ml

Anti-JMJD3 antibody used in Western Blot (WB). GTX31466

GTX31466 WB Image

WB analysis of HeLa cell lysate using GTX31466 JMJD3 antibody.
Working concentration : 0.25 μg/ml

REFERENCE

Application Reference

Chen L et al. J Immunol 2023; 210 (2) : 180-190 JMJD3 Is Required for Acute Pancreatitis and Pancreatitis-Associated Lung Injury.
Application : WB, IHC-Fr
Reactivity : Human, Mouse
Perrigue PM et al. Cancers (Basel) 2020; 12 (7) Cancer Stem Cell-Inducing Media Activates Senescence Reprogramming in Fibroblasts.
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399