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JMJD5 antibody

Anti-JMJD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX85251
Anti-JMJD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX85251
Anti-JMJD5 antibody used in Western Blot (WB). GTX85251

Cat. No. GTX85251

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all JMJD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Human Liver Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

JMJD5 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD5.The immunogen is located within amino acids 350 - 400 of JMJD5.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724205

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 8 , JMJD5

Cellular Localization

Nucleus

Background

The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD5 has not been identified. JMJD5 is expressed in multiple tissues.

Database

Research Area

DATA IMAGES

Anti-JMJD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX85251

GTX85251 IHC-P Image

IHC-P analysis of rat liver tissue using GTX85251 JMJD5 antibody.
Working concentration : 2.5 μg/ml

Anti-JMJD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX85251

GTX85251 IHC-P Image

IHC-P analysis of rat liver tissue using GTX85251 JMJD5 antibody.
Working concentration : 20 μg/ml

Anti-JMJD5 antibody used in Western Blot (WB). GTX85251

GTX85251 WB Image

WB analysis of human liver tissue lysate using GTX85251 JMJD5 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

Application Reference

Yang CY et al. PLoS One 2020; 15 (7) : e0236101 Downregulation of Jumonji-C domain-containing protein 5 inhibits proliferation by silibinin in the oral cancer PDTX model.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399