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JNK antibody

Anti-JNK antibody used in Western Blot (WB). GTX52360
Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52360
Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52360
Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52360
Anti-JNK antibody used in Western Blot (WB). GTX52360

Cat. No. GTX52360

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Pig
Package
100 μg ($399)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
Not tested in other applications.

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human JNK1/2/3, corresponding to a region within the internal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887797

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

JNK,JNK1,JNK1A2,JNK21B1/2,JNK46,MAPK8,PRKM8,SAPK1,SAPK1c,mitogenactivated protein kinase 8,JNK 1,mitogen-activated protein kinase 8,JNK2,JNK2A,JNK2ALPHA,JNK2B,JNK2BETA,JNK55,MAPK9,PRKM9,SAPK,SAPK1a,mitogenactivated protein kinase 9,p54a,p54aSAPK,JNK 2,mitogen-activated protein kinase 9,JNK3,JNK3A,MAPK10,PRKM10,SAPK1b,mitogenactivated protein kinase 10,p493F12,p54bSAPK,JNK 3,mitogen-activated protein kinase 10

Cellular Localization

Cytoplasm,Nucleus,Membrane,Mitochondrion

Database

Research Area

DATA IMAGES

Anti-JNK antibody used in Western Blot (WB). GTX52360

GTX52360 WB Image

WB analysis of mouse brain tissue lysate using GTX52360 JNK antibody. The lane on the left was treated with blocking peptide.

Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52360

GTX52360 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX52360 JNK antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52360

GTX52360 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX52360 JNK antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-JNK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX52360

GTX52360 ICC/IF Image

ICC/IF analysis of PFA-fixed LOVO cells using GTX52360 JNK antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400

Anti-JNK antibody used in Western Blot (WB). GTX52360

GTX52360 WB Image

WB analysis of hybridoma cell lysate using GTX52360 JNK antibody. The lane on the left was treated with blocking peptide.

REFERENCE

Application Reference

Zhang X et al. Chin Herb Med 2022; 14 (4) : 554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Li Y et al. Transl Lung Cancer Res 2021; 10 (4) : 1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Zhang X et al. Chin Herb Med 2022; 14 (4):554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.
Application : WB
Reactivity : Human
Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281 Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling.
Chen CF et al. Oncol Lett 2018; 16 (5):6323-6330 Synergistic inhibitory effects of cetuximab and curcumin on human cisplatin-resistant oral cancer CAR cells through intrinsic apoptotic process.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399