JNK (phospho Thr183/Tyr185) antibody [HL1008]

Cat. No. GTX635799

Reference 8

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1008
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all JNK products

Datasheet
Reference

View product citations for antibody GTX635799 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent

Not tested in other applications.

Observed MW

46, 54 kDa.

Product Note

This antibody detects JNK when dually phosphorylated at Thr183 and Tyr185, and singly phosphorylated at Thr183.

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr183/Tyr185 of human JNK. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888579

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Database

Gene ID: 5599 MAPK8
UniProt: P45983 MAPK8

Research Area

DATA IMAGES

GTX635799 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX635799 WB Image

Untreated (–) and treated (+) 293T whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635799 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635799 WB Image

Untreated (–) and treated (+) PC-12 whole cell extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635799 IHC-P Image

JNK (phospho Thr183/Tyr185) antibody [HL1008] detects JNK (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK (phospho Thr183/Tyr185) stained by JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635799 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

E Prosdocimi et al. Sci Adv 2024; 10 (36) : eadn9361 BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway

PY Tsai et al. Biochem Pharmacol 2024; 227 : 116423 Aspirin alleviates fibronectin-induced preeclampsia phenotypes in a mouse model and reverses fibronectin-mediated trophoblast invasiveness under hypoxia by regulating ciliogenesis and Akt and MAPK signaling

Application : WB

Reactivity : Human

Chien LH et al. Antioxidants (Basel) 2021; 10 (10)

Application : WB

Reactivity : Mouse

Jiang WP et al. Antioxidants (Basel) 2021; 10 (6) Sanghuangporus sanghuang Mycelium Prevents Paracetamol-Induced Hepatotoxicity through Regulating the MAPK/NF-κB, Keap1/Nrf2/HO-1, TLR4/PI3K/Akt, and CaMKKβ/LKB1/AMPK Pathways and Suppressing Oxidative Stress and Inflammation.

Application : WB

Reactivity : Mouse

Li Y et al. Transl Lung Cancer Res 2021; 10 (4) : 1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.

Application : WB

Reactivity : Human

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REVIEW

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Application Reference

E Prosdocimi et al. Sci Adv 2024; 10 (36):eadn9361 BioID-based intact cell interactome of the Kv1.3 potassium channel identifies a Kv1.3-STAT3-p53 cellular signaling pathway

PY Tsai et al. Biochem Pharmacol 2024; 227:116423 Aspirin alleviates fibronectin-induced preeclampsia phenotypes in a mouse model and reverses fibronectin-mediated trophoblast invasiveness under hypoxia by regulating ciliogenesis and Akt and MAPK signaling

Application : WB

Reactivity : Human

Chien LH et al. Antioxidants (Basel) 2021; 10 (10)

Application : WB

Reactivity : Mouse

Application : WB

Reactivity : Mouse

Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.

Application : WB

Reactivity : Human

Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.

Application : WB

Reactivity : Human

Mandal JP et al. Free Radic Biol Med 2021; 163:69-87 PKCδ mediates mitochondrial ROS generation and oxidation of HSP60 to relieve RKIP inhibition on MAPK pathway for HCC progression.

Application : WB

Reactivity : Human

Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.

Application : WB

Reactivity : Human

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

JNK (phospho Thr183/Tyr185) antibody [HL1008]

Cat. No. GTX635799

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Observed MW

Product Note

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX635799 WB Image

GTX635799 WB Image

GTX635799 WB Image

GTX635799 WB Image

GTX635799 IHC-P Image

GTX635799 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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