*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody is raised against JNK1/2 phosphorylated at Thr183/Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221/Tyr223. The antibody has been shown to recognize the endogenous, active forms of JNK 1 + 2 in a variety of cell types following treatment by a broad range of extracellular stimuli [e.g. including 293 cells (human embryonic kidney; +/- ultraviolet light) and PC12 cells (rat pheochromocytoma; +/- sorbital)].
PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JNK1 and 2 that contains threonine 183 and tyrosine 185. This region is conserved among many species including mouse, rat, chicken, nematode, fruit fly, and in JNK3.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. The jun family member, cJun, is phosphorylated by another kinase termed cJun N-terminal kinase (JNK).