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JNK (phospho Thr183/Tyr185) antibody

Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24821
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX24821

Cat No. GTX24821

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, IHC

Reactivity

Human, Mouse, Rat, Pig, Primate
Package
50 μl ($259)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:2000
ICC/IF 1:250
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody is raised against JNK1/2 phosphorylated at Thr183/Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221/Tyr223. The antibody has been shown to recognize the endogenous, active forms of JNK 1 + 2 in a variety of cell types following treatment by a broad range of extracellular stimuli [e.g. including 293 cells (human embryonic kidney; +/- ultraviolet light) and PC12 cells (rat pheochromocytoma; +/- sorbital)].

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JNK1 and 2 that contains threonine 183 and tyrosine 185. This region is conserved among many species including mouse, rat, chicken, nematode, fruit fly, and in JNK3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. The jun family member, cJun, is phosphorylated by another kinase termed cJun N-terminal kinase (JNK).

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24821

GTX24821 ICC/IF Image

ICC/IF analysis of A549 cells treated with Anisomycin (25 μg/mL for 30 min) using GTX24821 JNK (phospho Thr183/Tyr185) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX24821

GTX24821 WB Image

WB analysis of various samples using GTX24821 JNK (phospho Thr183/Tyr185) antibody.
Dilution : 1:1000
Lane 1: HEK-293
Lane 2: HEK-293 treated for 5 minutes with 200 mM of Anisomycin
Lane 3: HEK-293 treated for 20 minutes with UV
Lane 4: MCF7
Lane 5: MCF7 treated for 5 minutes with 200 mM of Anisomycin
Lane 6: K562
Lane 7: K562 treated for 20 minutes with UV
Lane 8: HeLa
Lane 9: HeLa treated for 20 minutes with UV
Loading : 20ug

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 259