ICC/IF analysis of A549 cells treated with Anisomycin (25 μg/mL for 30 min) using GTX24821 JNK (phospho Thr183/Tyr185) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

WB analysis of various samples using GTX24821 JNK (phospho Thr183/Tyr185) antibody.
Dilution : 1:1000
Lane 1: HEK-293
Lane 2: HEK-293 treated for 5 minutes with 200 mM of Anisomycin
Lane 3: HEK-293 treated for 20 minutes with UV
Lane 4: MCF7
Lane 5: MCF7 treated for 5 minutes with 200 mM of Anisomycin
Lane 6: K562
Lane 7: K562 treated for 20 minutes with UV
Lane 8: HeLa
Lane 9: HeLa treated for 20 minutes with UV
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