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JNK (phospho Thr183/Tyr185) antibody

Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50868
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

Cat No. GTX50868

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Specificity/Sensitivity

This antibody is raised against JNK1/2 phosphorylated at Thr183/Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221/Tyr223.

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from human JNK.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 8 , mitogen-activated protein kinase 9 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK

Cellular Localization

Cytoplasm,Nucleus,Membrane,Mitochondrion

Database

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50868

GTX50868 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50868 JNK (phospho Thr183/Tyr185) antibody.

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

GTX50868 WB Image

WB analysis of extracts from C6 cells untreated or treated with anisomycin using GTX50868 JNK (phospho Thr183/Tyr185) antibody.

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

GTX50868 WB Image

WB analysis of extracts from 293 cells treated with Anisomycin or calf intestinal phosphatase (CIP) using GTX50868 JNK (phospho Thr183/Tyr185) antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf