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JNK (phospho Thr183/Tyr185) antibody

Orthogonal
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868
Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50868
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

Cat. No. GTX50868

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Product Note

This antibody is raised against JNK1/2 phosphorylated at Thr183/Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221/Tyr223.

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from human JNK.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11172628

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 , mitogen-activated protein kinase 9 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK

Cellular Localization

Cytoplasm,Nucleus,Membrane,Mitochondrion

Database

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

GTX50868 WB Image

WB analysis of extracts from C6 cells untreated or treated with anisomycin using GTX50868 JNK (phospho Thr183/Tyr185) antibody.

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

GTX50868 WB Image

WB analysis of extracts from 293 cells treated with Anisomycin or calf intestinal phosphatase (CIP) using GTX50868 JNK (phospho Thr183/Tyr185) antibody.

Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50868

GTX50868 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50868 JNK (phospho Thr183/Tyr185) antibody.

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX50868

GTX50868 WB Image

Orthogonal

The data was published in the 2019 in Antioxidants (Basel). PMID: 31906147

REFERENCE

Application Reference

Chang WT et al. Oxid Med Cell Longev 2021; 2021 : 7689045 A Marine Terpenoid, Heteronemin, Induces Both the Apoptosis and Ferroptosis of Hepatocellular Carcinoma Cells and Involves the ROS and MAPK Pathways.
Application : WB
Reactivity : Human
Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399