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JNK (phospho Thr183/Tyr185) antibody

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX52326
Anti-JNK (phospho Thr183/Tyr185) antibody used in IHC (Paraffin sections) (IHC-P). GTX52326

Cat. No. GTX52326

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all JNK products

APPLICATION

Application Note

WB 1:500-1:2000

Calculated MW

48 kDa. ( Note )

Product Note

This antibody is raised against JNK1/2 phosphorylated at Thr183/Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221/Tyr223.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human JNK around the phosphorylation site of Thr183/Tyr185.

Purification

Antigen affinity purification

Conjugation

Unconjugated

RRID

AB_2887791

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX52326

GTX52326 WB Image

Western blot analysis of UV treated 293 whole cell lysate using GTX52326 JNK1/2/3 (phospho Thr183/Tyr185) antibody. The lane on the left is treated with the antigen-specific peptide.

Anti-JNK (phospho Thr183/Tyr185) antibody used in IHC (Paraffin sections) (IHC-P). GTX52326

GTX52326 IHC-P Image

IHC-P analysis of human brain tissue using GTX52326 JNK1/2/3 (phospho Thr183/Tyr185) antibody.

REFERENCE

Application Reference

Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281
Chen CF et al. Oncol Lett 2018; 16 (5) : 6323-6330
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399