APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:1000 |
1:100 - 1:500 |
1:100 - 1:200 |
Not tested in other applications.
Product Note
This antibody is raised against JNK1/2 phosphorylated at Thr183. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221.
PROPERTIES
Form
Liquid
Buffer
0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of JNK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 8 , mitogen-activated protein kinase 9 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK
Cellular Localization
Cytoplasm,Nucleus,Membrane,Mitochondrion
Database
Research Area
DATA IMAGES
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GTX34387 WB Image
WB analysis of PMA-treated MCF7 (A), UV-treated NIH3T3 (B), PMA-treated PC12 (C) whole cell lysates using GTX34387 JNK (phospho Thr183) antibody.
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GTX34387 ICC/IF Image
ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX34387 JNK (phospho Thr183) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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GTX34387 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX34387 JNK (phospho Thr183) antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
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REFERENCE
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REVIEW
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