GeneTex
United States (US)

JNK (phospho Thr183) antibody

Anti-JNK (phospho Thr183) antibody used in IHC (Paraffin sections) (IHC-P). GTX34387
Anti-JNK (phospho Thr183) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34387
Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX34387

Cat No. GTX34387

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Specificity/Sensitivity

This antibody is raised against JNK1/2 phosphorylated at Thr183. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221.
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of JNK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-JNK (phospho Thr183) antibody used in IHC (Paraffin sections) (IHC-P). GTX34387

GTX34387 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX34387 JNK (phospho Thr183) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-JNK (phospho Thr183) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34387

GTX34387 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX34387 JNK (phospho Thr183) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX34387

GTX34387 WB Image

WB analysis of PMA-treated MCF7 (A), UV-treated NIH3T3 (B), PMA-treated PC12 (C) whole cell lysates using GTX34387 JNK (phospho Thr183) antibody.

Package List Price ($)
$ 319