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JNK (phospho Thr183) antibody

Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX50278
Anti-JNK (phospho Thr183) antibody used in IHC (Paraffin sections) (IHC-P). GTX50278
Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX50278

Cat No. GTX50278

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Specificity/Sensitivity

This antibody is raised against JNK1/2 phosphorylated at Thr183. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from human JNK.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 8 , mitogen-activated protein kinase 9 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK

Cellular Localization

Cytoplasm,Nucleus,Membrane,Mitochondrion

Database

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX50278

GTX50278 WB Image

WB analysis of extracts from 293 cells using GTX50278 JNK (phospho Thr183) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

Anti-JNK (phospho Thr183) antibody used in IHC (Paraffin sections) (IHC-P). GTX50278

GTX50278 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50278 JNK (phospho Thr183) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-JNK (phospho Thr183) antibody used in Western Blot (WB). GTX50278

GTX50278 WB Image

WB analysis of extracts from 293 cells treated with UV or calf intestinal phosphatase (CIP) using GTX50278 JNK (phospho Thr183) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf