*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody is raised against JNK1/2 phosphorylated at Thr183. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221.
Mouse, Rat(>80% identity)
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from human JNK.
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
mitogen-activated protein kinase 8 , mitogen-activated protein kinase 9 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK
GTX50278 WB Image
WB analysis of extracts from 293 cells using GTX50278 JNK (phospho Thr183) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide
GTX50278 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50278 JNK (phospho Thr183) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
GTX50278 WB Image
WB analysis of extracts from 293 cells treated with UV or calf intestinal phosphatase (CIP) using GTX50278 JNK (phospho Thr183) antibody.