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JNK (phospho Tyr185) antibody [EP1597Y]

Anti-JNK (phospho Tyr185) antibody [EP1597Y] used in Western Blot (WB). GTX61742

Cat. No. GTX61742

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EP1597Y

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all JNK products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1,000
For IP: Use at a dilution of 1:20
Optimal working dilution for a specific application should be determined by the investigator.

Calculated MW

46 kDa. ( Note )

Product Note

This antibody is raised against JNK1/2 phosphorylated at Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Tyr223.

PROPERTIES

Form

Liquid

Buffer

50mM Tris-Glycine, 150mM NaCl, 0.05% BSA, 40% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of human JNK3 was used as immunogen.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.

TARGET

Synonyms

Mitogen-Activated Protein Kinase 8 , Jnk , Jnk-46 , Jnk1 , Jnk1A2 , Jnk21B1/2 , Prkm8 , Sapk1 , Sapk1C , Mapk8

Background

C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c . JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 . Unlike other JNK isoforms, JNK3 is only expressed in the heart and nervous system. JNK3 has been implicated in stress-induced neuronal apoptosis .

Research Area

DATA IMAGES

Anti-JNK (phospho Tyr185) antibody [EP1597Y] used in Western Blot (WB). GTX61742

GTX61742 WB Image

A. Western blot analysis on Hek293 cell lysates using anti-JNK3 Phospho (pS223) RabMAb (cat. #GTX61742) 1:20000 dilution. Cells were either (A) untreated (B) treated with UV.

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399