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JNK (phospho Tyr185) antibody

Anti-JNK (phospho Tyr185) antibody used in Western Blot (WB). GTX52328

Cat No. GTX52328

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

This antibody is raised against JNK1/2 phosphorylated at Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Tyr223.

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human JNK around the phosphorylation site of Tyr185

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Mitogen-Activated Protein Kinase 8 , Jnk , Jnk-46 , Jnk1 , Jnk1A2 , Jnk21B1/2 , Prkm8 , Sapk1 , Sapk1C , Mapk8

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JNK (phospho Tyr185) antibody used in Western Blot (WB). GTX52328

GTX52328 WB Image

Western blot analysis of SAPK/JNK phosphorylation expression in nocodazole treated HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf