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JNK1 + JNK2 antibody

Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806
Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806
Anti-JNK1 + JNK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133806

Cat No. GTX133806

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , SK-N-AS , rat brain , JNK1 and JNK2-transfected 293T

Predict Reactivity

Mouse, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human JNK1 + JNK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887115

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

JNK1 antibody , JNK1A2 antibody , JNK21B1/2 antibody , JNK46 antibody , MAP kinase 8 antibody , MAPK 8 antibody , MAPK8 antibody , PRKM8 antibody , SAPK1 antibody , SAPK1c antibody , mitogen-activated protein kinase 8 antibody

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806

GTX133806 WB Image

Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-JNK1 + JNK2 antibody used in Western Blot (WB). GTX133806

GTX133806 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-JNK1 + JNK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133806

GTX133806 IHC-P Image

JNK1 + JNK2 antibody detects JNK1 + JNK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK1 + JNK2 stained by JNK1 + JNK2 antibody (GTX133806) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169