GeneTex
United States (US)

JNK1 + JNK2 antibody

Overexpression
GTX133806_43089_20180126_WB_H_R_18121410_766.jpg
GTX133806_43089_20180126_WB_B_18121410_226.jpg
GTX133806_43089_20180306_IHC-P_18121410_572.jpg

Cat No. GTX133806

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , SK-N-AS , rat brain , JNK1 and JNK2-transfected 293T

Predict Reactivity

Mouse, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human JNK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

JNK1 antibody , JNK1A2 antibody , JNK21B1/2 antibody , JNK46 antibody , MAP kinase 8 antibody , MAPK 8 antibody , MAPK8 antibody , PRKM8 antibody , SAPK1 antibody , SAPK1c antibody , mitogen-activated protein kinase 8 antibody

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Research Area

DATA IMAGES
GTX133806_43089_20180126_WB_H_R_18121410_766.jpg

GTX133806 WB Image

Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133806_43089_20180126_WB_B_18121410_226.jpg

GTX133806 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133806_43089_20180306_IHC-P_18121410_572.jpg

GTX133806 IHC-P Image

JNK1 + JNK2 antibody detects JNK1 + JNK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK1 + JNK2 stained by JNK1 + JNK2 antibody (GTX133806) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf