APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Product Note
This antibody can detect JNK1 and JNK3 protein, and it does not cross react with JNK2 protein based on internal testing.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human JNK1/3
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 8 , mitogen-activated protein kinase 10 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c , JNK3 , JNK3A , PRKM10 , SAPK1b , p493F12 , p54bSAPK
Cellular Localization
Cytoplasm , Nucleus , Membrane , Mitochondrion
Database
DATA IMAGES
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/templates/default/js/lightbox/images/loading.gif "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
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GTX02824 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK3 antibody [GT1227] (GTX02824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX02824 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK3 antibody [GT1227] (GTX02824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX02824 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK3 antibody [GT1227] (GTX02824) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX02824 IHC-P Image
IHC-P analysis of human colon tissue section using GTX02824 JNK1 + JNK3 antibody [GT1227].
Dilution : 1:100
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GTX02824 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX02824 JNK1 + JNK3 antibody [GT1227].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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GTX02824 ICC/IF Image
ICC/IF analysis of C6 cells using GTX02824 JNK1 + JNK3 antibody [GT1227].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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GTX02824 WB Image
WB analysis of various samples using GTX02824 JNK1 + JNK3 antibody [GT1227].
Dilution : 1:1000
Loading : 25μg
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GTX02824 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX02824 JNK1 + JNK3 antibody [GT1227].
Dilution : 1:100
Blue : DAPI for nuclear staining.
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REFERENCE
There are currently no references for JNK1 + JNK3 antibody [GT1227] (GTX02824). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for JNK1 + JNK3 antibody [GT1227] (GTX02824). Be the first to share your experience with this product.
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/small/GTX02824/GTX02824_4000001163_20210122_WB_w_23053123_100.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/small/GTX02824/GTX02824_4000001163_20210122_WB_B_w_23053123_709.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/small/GTX02824/GTX02824_4000001163_20210122_WB_2_w_23053123_918.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in IHC (Paraffin sections) (IHC-P). GTX02824](/upload/website/prouct_img/small/GTX02824/A5051_IHC_01_(1072008)_w_23053123_637.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in IHC (Paraffin sections) (IHC-P). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824](/upload/website/prouct_img/small/GTX02824/A5051_IF_02_(1073525)_w_23053123_223.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824](/upload/website/prouct_img/small/GTX02824/A5051_IF_01_(1073524)_w_23053123_520.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/small/GTX02824/CutImage_A5051_WB_01_(1070831)_w_23053123_570.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824](/upload/website/prouct_img/small/GTX02824/A5051_IF_03_(1073526)_w_23053123_784.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/normal/GTX02824/GTX02824_4000001163_20210122_WB_w_23053123_100.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/normal/GTX02824/GTX02824_4000001163_20210122_WB_B_w_23053123_709.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/normal/GTX02824/GTX02824_4000001163_20210122_WB_2_w_23053123_918.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in IHC (Paraffin sections) (IHC-P). GTX02824](/upload/website/prouct_img/normal/GTX02824/A5051_IHC_01_(1072008)_w_23053123_637.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in IHC (Paraffin sections) (IHC-P). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824](/upload/website/prouct_img/normal/GTX02824/A5051_IF_02_(1073525)_w_23053123_223.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824](/upload/website/prouct_img/normal/GTX02824/A5051_IF_01_(1073524)_w_23053123_520.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824](/upload/website/prouct_img/normal/GTX02824/CutImage_A5051_WB_01_(1070831)_w_23053123_570.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Western Blot (WB). GTX02824")
![Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824](/upload/website/prouct_img/normal/GTX02824/A5051_IF_03_(1073526)_w_23053123_784.webp "Anti-JNK1 + JNK3 antibody [GT1227] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02824")