GeneTex

  • Country / Location Selection

United States (US)

JNK1 antibody [1C2]

Overexpression
Anti-JNK1 antibody [1C2] used in Western Blot (WB). GTX84143
Anti-JNK1 antibody [1C2] used in Western Blot (WB). GTX84143
Anti-JNK1 antibody [1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84143
Anti-JNK1 antibody [1C2] used in Western Blot (WB). GTX84143

Cat. No. GTX84143

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1C2

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-384 of human JNK1 (NP_002741) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c

Cellular Localization

Cytoplasm,Nucleus

Background

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Database

Research Area

DATA IMAGES

Anti-JNK1 antibody [1C2] used in Western Blot (WB). GTX84143

GTX84143 WB Image

WB analysis of various cell lines using GTX84143 JNK1 antibody [1C2].
Loading : 35 ug per lane

Anti-JNK1 antibody [1C2] used in Western Blot (WB). GTX84143

GTX84143 WB Image

WB analysis of NIH/3T3 cell lysate using GTX84143 JNK1 antibody [1C2].
Dilution : 1:1000

Anti-JNK1 antibody [1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84143

GTX84143 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with JNK1 plasmid using GTX84143 JNK1 antibody [1C2].

Anti-JNK1 antibody [1C2] used in Western Blot (WB). GTX84143

GTX84143 WB Image

Overexpression

WB analysis of HEK293T cells transfected with JNK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84143 JNK1 antibody [1C2].
Loading : 5 ug per lane

REFERENCE

There are currently no references for JNK1 antibody [1C2] (GTX84143). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for JNK1 antibody [1C2] (GTX84143). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519