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JNK2 antibody [N1C3]

Overexpression
Anti-JNK2 antibody [N1C3] used in Western Blot (WB). GTX105523
Anti-JNK2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105523
Anti-JNK2 antibody [N1C3] used in Western Blot (WB). GTX105523
Anti-JNK2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105523
Anti-JNK2 antibody [N1C3] used in Western Blot (WB). GTX105523
Anti-JNK2 antibody [N1C3] used in Immunohistochemistry (IHC). GTX105523

Cat. No. GTX105523

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Plutella xylostella
Package
100 μl ($399),
25 μl ($169)
See all JNK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

MCF-7

Product Note

This antibody may cross with JNK1 or JNK3.

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human JNK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950882

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 9 , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-JNK2 antibody [N1C3] used in Western Blot (WB). GTX105523

GTX105523 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX105523 diluted at 1:1000

Anti-JNK2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105523

GTX105523 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using MAPK9(GTX105523) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-JNK2 antibody [N1C3] used in Western Blot (WB). GTX105523

GTX105523 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] (GTX105523) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-JNK2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105523

GTX105523 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using JNK2(GTX105523) antibody at 1:200 dilution.

Anti-JNK2 antibody [N1C3] used in Western Blot (WB). GTX105523

GTX105523 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] (GTX105523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-JNK2 antibody [N1C3] used in Immunohistochemistry (IHC). GTX105523

GTX105523 IHC Image

The data was published in the journal PLoS Pathog in 2014. PMID: 25144637

REFERENCE

Application Reference

Chuang YF et al. PLoS One 2015; 10 (8) : e0137177 Protein Phosphatase 2A in Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Murine Lymphatic Endothelial Cells.
Application : WB
Reactivity : Mouse
Chen WJ et al. PLoS Pathog 2014; 10 (8) : e1004288 Characterization of an Insecticidal Toxin and Pathogenicity of Pseudomonas taiwanensis against Insects.
Application : IHC-P
Reactivity : Plutella xylostella
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169