Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] (GTX105523) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed MCF-7, using JNK2(GTX105523) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] (GTX105523) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using MAPK9(GTX105523) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX105523 diluted at 1:1000

The data was published in the journal PLoS Pathog in 2014. PMID: 25144637