APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human MAPK9 (NP_002743.3).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase 9 , JNK-55 , JNK2 , JNK2A , JNK2ALPHA , JNK2B , JNK2BETA , PRKM9 , SAPK , SAPK1a , p54a , p54aSAPK
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]
Database
Research Area
DATA IMAGES
|
GTX32689 WB Image
WB analysis of various sample lysates using GTX32689 JNK2 antibody.
Dilution : 1:1000
Loading : 25μg per lane
|
|
|
GTX32689 WB Image
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX32689 JNK2 antibody.
Dilution : 1:1000
Loading : 25μg per lane
|
|
|
GTX32689 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX32689 JNK2 antibody.
Dilution : 1:100
|
|
|
GTX32689 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX32689 JNK2 antibody.
Blue : DAPI
|
|
|
GTX32689 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX32689 JNK2 antibody.
Dilution : 1:100
|
REFERENCE
There are currently no references for JNK2 antibody (GTX32689). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for JNK2 antibody (GTX32689). Be the first to share your experience with this product.
