GeneTex
United States (US)

JNK2 antibody

KO/KD
GTX32689_20180530_ICCIF_18121410_983.jpg
GTX32689_20180530_IHC-P_1_18121410_658.jpg
GTX32689_20180530_IHC-P_18121410_679.jpg
GTX32689_20180822_WB_18121410_908.jpg
GTX32689_20180530_WB_18121410_317.jpg

Cat No. GTX32689

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human MAPK9 (NP_002743.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase 9,Jnk-55,Jnk2,Jnk2A,Jnk2Alpha,Jnk2B,Jnk2Beta,Prkm9,Sapk,Sapk1A,P54A,P54Asapk,Mapk9

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES
GTX32689_20180530_ICCIF_18121410_983.jpg

GTX32689 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX32689 JNK2 antibody.
Blue : DAPI

GTX32689_20180530_IHC-P_1_18121410_658.jpg

GTX32689 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32689 JNK2 antibody.
Dilution : 1:100

GTX32689_20180530_IHC-P_18121410_679.jpg

GTX32689 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX32689 JNK2 antibody.
Dilution : 1:100

GTX32689_20180822_WB_18121410_908.jpg

GTX32689 WB Image

KO/KD

WB analysis of wild type and JNK2 knockout cell lysate using GTX32689 JNK2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32689_20180530_WB_18121410_317.jpg

GTX32689 WB Image

WB analysis of various samples using GTX32689 JNK2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329