APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , Daudi Cell Lysate(GTX23951)
Product Note
This antibody is specific for JPH3 C-Terminus
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
JPH3 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human JPH3.The immunogen is located within amino acids 540 - 590 of JPH3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
junctophilin 3 , CAGL237 , HDL2 , JP-3 , JP3 , TNRC22
Cellular Localization
Cell membrane,Endoplasmic reticulum membrane
Background
Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain. In the CNS, both JPH3 and JPH4 are expressed throughout neural sites and contribute to the subsurface cistern formation in neurons. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. At least two isoforms of JPH3 are known to exist.
Database
Research Area
DATA IMAGES
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GTX85366 WB Image
WB analysis of Daudi cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85366 JPH3 antibody.
Working concentration : 1 μg/ml
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GTX85366 IHC-P Image
IHC-P analysis of human brain tissue using GTX85366 JPH3 antibody.
Working concentration : 20 μg/ml
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GTX85366 IHC-P Image
IHC-P analysis of human brain tissue using GTX85366 JPH3 antibody.
Working concentration : 2.5 μg/ml
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REFERENCE
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REVIEW
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