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Jab1 antibody [2A10.8]

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203
Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203
Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203
Anti-Jab1 antibody [2A10.8] used in Immunohistochemistry (IHC). GTX70203

Cat No. GTX70203

Host

Mouse

Clonality

Monoclonal

Clone Name

2A10.8

Isotype

IgG2b

Application

WB, ICC/IF, IP, IHC

Reactivity

Human, Mouse, Rat, Drosophila
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP 0.5 - 1 μg/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , MCF-7 , MDA-MB-231 , Rat liver

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

The full-length mouse JAB-1 gene expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cop9 Signalosome Subunit 5 , Csn5 , Jab1 , Mov-34 , Sgn5 , Cops5

Cellular Localization

Nuclear/Cytoplasmic

Background

JAB-1 is a 38 kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the ""Signalosome"", which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome.

Database

Research Area

DATA IMAGES

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

GTX70203 WB Image

Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 antibody (GTX70203) diluted at a dilution of 1:500.

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

GTX70203 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX70203 diluted at 1:10000

Anti-Jab1 antibody [2A10.8] used in Western Blot (WB). GTX70203

GTX70203 WB Image

Sample (50 ug of whole cell lysate)
A: Rat liver
10% SDS PAGE
GTX70203 diluted at 1:1000

Anti-Jab1 antibody [2A10.8] used in Immunohistochemistry (IHC). GTX70203

GTX70203 IHC Image

REFERENCE

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Application Reference
SDS
PBS.pdf