Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113927 diluted at 1:1000

JAB1 antibody [N1C1] detects JAB1 protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
JAB1 antibody [N1C1] (GTX113927) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

JAB1 antibody [N1C1] detects JAB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: JAB1 protein stained by JAB1 antibody [N1C1] (GTX113927) diluted at 1:200.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX113927 diluted at 1:1000

Sample (30 μg of whole cell lysate)
A: Zebrafish eye
10% SDS PAGE
GTX113927 diluted at 1:1000

Jab1 antibody [N1C1] detects Jab1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Jab1 antibody [N1C1] (GTX113927) dilution: 1:100.