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Jab1 antibody [N1C1]

Anti-Jab1 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX113927
Anti-Jab1 antibody [N1C1] used in Western Blot (WB). GTX113927
Anti-Jab1 antibody [N1C1] used in Western Blot (WB). GTX113927
Anti-Jab1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113927
Anti-Jab1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113927
Anti-Jab1 antibody [N1C1] used in Western Blot (WB). GTX113927

Cat. No. GTX113927

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Jab1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Mouse brain , HeLa

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Jab1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036644

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COP9 signalosome subunit 5 , CSN5 , JAB1 , MOV-34 , SGN5

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Jab1 antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX113927

GTX113927 IHC-Wm Image

Jab1 antibody [N1C1] detects Jab1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Jab1 antibody [N1C1] (GTX113927) dilution: 1:100.
Anti-Jab1 antibody [N1C1] used in Western Blot (WB). GTX113927

GTX113927 WB Image

Sample (30 μg of whole cell lysate)
A: Zebrafish eye
10% SDS PAGE
GTX113927 diluted at 1:1000

Anti-Jab1 antibody [N1C1] used in Western Blot (WB). GTX113927

GTX113927 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX113927 diluted at 1:1000

Anti-Jab1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113927

GTX113927 ICC/IF Image

JAB1 antibody [N1C1] detects JAB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: JAB1 protein stained by JAB1 antibody [N1C1] (GTX113927) diluted at 1:200.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.
Anti-Jab1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113927

GTX113927 IHC-P Image

JAB1 antibody [N1C1] detects JAB1 protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
JAB1 antibody [N1C1] (GTX113927) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Jab1 antibody [N1C1] used in Western Blot (WB). GTX113927

GTX113927 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX113927 diluted at 1:1000

REFERENCE

Application Reference

Huang X et al. openbio 2016; (Epub) Overexpression of COP9 signalosome subunits, CSN7A and CSN7B, exerts different effects on adipogenic differentiation.
Application : WB
Reactivity : Human
Xu GP et al. Biochem Biophys Res Commun 2013; 432 (3) : 425-30 Rig-G negatively regulates SCF-E3 ligase activities by disrupting the assembly of COP9 signalosome complex.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169