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Jagged 1 antibody

Anti-Jagged 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX48691
Anti-Jagged 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48691
Anti-Jagged 1 antibody used in Western Blot (WB). GTX48691

Cat. No. GTX48691

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Jagged 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:200 - 1:1,000
IHC-P 1:100 - 1:500
ELISA 1:20,000 - 1:100,000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176335

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

jagged canonical Notch ligand 1 , AGS , AGS1 , AHD , AWS , CD339 , DCHE , HJ1 , JAGL1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies

Database

Research Area

DATA IMAGES

Anti-Jagged 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX48691

GTX48691 IHC-P Image

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using GeneTex's Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.

Anti-Jagged 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48691

GTX48691 ICC/IF Image

Immunofluorescence Microscopy using GeneTex's Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 Green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue)

Anti-Jagged 1 antibody used in Western Blot (WB). GTX48691

GTX48691 WB Image

WB analysis of mouse liver using GTX48691Jagged 1 antibody at 1:500.

REFERENCE

Application Reference

Zhu Q et al. Oncol Lett 2020; 19 (1) : 103-112 Linc-OIP5 loss regulates migration and invasion in MDA-MB-231 breast cancer cells by inhibiting YAP1/JAG1 signaling.
Zhu Q et al. Biol Res 2020; 53 (1) : 5 Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor microenvironment.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399