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Jagged 1 antibody

Anti-Jagged 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX48691
Anti-Jagged 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48691
Anti-Jagged 1 antibody used in Western Blot (WB). GTX48691

Cat No. GTX48691

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:200 - 1:1,000
IHC-P 1:100 - 1:500
ELISA 1:20,000 - 1:100,000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176335

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

jagged canonical Notch ligand 1 , AGS , AGS1 , AHD , AWS , CD339 , DCHE , HJ1 , JAGL1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies

Database

Research Area

DATA IMAGES

Anti-Jagged 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX48691

GTX48691 IHC-P Image

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using GeneTex's Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.

Anti-Jagged 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48691

GTX48691 ICC/IF Image

Immunofluorescence Microscopy using GeneTex's Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 Green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue)

Anti-Jagged 1 antibody used in Western Blot (WB). GTX48691

GTX48691 WB Image

WB analysis of mouse liver using GTX48691Jagged 1 antibody at 1:500.

REFERENCE

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SDS
Sodium Azide.pdf