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Japanese encephalitis virus Envelope antibody

Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX125867
Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

Cat. No. GTX125867

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-P (cell pellet)

Reactivity

Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Japanese encephalitis virus infected BHK-21 cells

Product Note

This antibody is specific for JEV Envelope protein, and it does not cross-react with Zika, DENV-1, DENV-2, DENV-3, and DENV-4 Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Envelope protein (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172365

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Envelope , JEV Envelope

Cellular Localization

Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

GTX125867 ICC/IF Image

Envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in MeOH.
Green: envelope protein (Japanese encephalitis virus) protein stained by Envelope protein (Japanese encephalitis virus) antibody (GTX125867) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Japanese encephalitis virus Envelope antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125867

GTX125867 ICC/IF Image

Envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis viruswere fixed in paraformaldehyde.
Green: envelope protein (Japanese encephalitis virus) protein stained by Envelope protein (Japanese encephalitis virus) antibody (GTX125867) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

GTX125867 WB Image

envelope protein (Japanese encephalitis virus) antibody detects envelope protein (Japanese encephalitis virus) protein by western blot analysis.
A. 10 μg BHK-21 whole cell extract
B. 10 μg whole cell extract of Japanese encephalitis virus infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
10% SDS-PAGE
envelope protein (Japanese encephalitis virus) antibody (GTX125867) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Japanese encephalitis virus Envelope antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX125867

GTX125867 IHC-P (cell pellet) Image

Envelope protein (JEV) antibody detects Envelope protein (JEV) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded japanese encephalitis virus BHK-21.
Green: Envelope protein (JEV) stained by Envelope protein (JEV) antibody (GTX125867) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Japanese encephalitis virus Envelope antibody used in Western Blot (WB). GTX125867

GTX125867 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Japanese encephalitis virus Envelope antibody (GTX125867) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

Japanese encephalitis virus Envelope antibody Cat. No. GTX125867

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 26-Aug-2014
Score :
Application Tested : IHC-P
Sample Species : JEV
Sample : Japanese encephalitis virus infected mouse brain
Blocking : Normal Goat Serum Blocking in TBS, 25°C, 0.3Hr
Fixation : 10% Formalin
Permeabilization /Antigen retrieval : TE buffer
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169