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Japanese encephalitis virus NS1' antibody

Anti-Japanese encephalitis virus NS1' antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132499
Anti-Japanese encephalitis virus NS1' antibody used in Western Blot (WB). GTX132499

Cat. No. GTX132499

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus NS1' products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:5000
Not tested in other applications.

Positive Control

JEV infected BHK21 cells

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of NS1' (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886663

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Non-structural protein 1' , Japanese encephalitis Virus NS1' , JEV Non-structural protein 1' , JEV NS1'

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus NS1' antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132499

GTX132499 ICC/IF Image

JEV NS1' antibody detects JEV NS1' protein by immunofluorescent analysis. Sample: BHK-21 cells mock (left) and infected with JEV (right) were fixed in 4% paraformaldehyde. Green: JEV NS1' protein stained by JEV NS1' antibody (GTX132499) diluted at 1:5000. Blue: DAPI staining.

Anti-Japanese encephalitis virus NS1' antibody used in Western Blot (WB). GTX132499

GTX132499 WB Image

Non-infected (–) and infected (+) BHK-21 cell extracts (30 μg) were separated by 10% SDS-PAGE. Left: the membrane was blotted with JEV NS1 antibody (GTX131370) diluted at 1:5000; Right: the membrane was blotted with JEV NS1' antibody (GTX132499) diluted at 1:20000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169