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Japanese encephalitis virus NS4A antibody

Anti-Japanese encephalitis virus NS4A antibody used in Western Blot (WB). GTX132028

Cat. No. GTX132028

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus NS4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

JEV-infected BHK-21 cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of NS4A (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886547

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Non-structural protein 4A , Japanese encephalitis Virus NS4A , JEV Non-structural protein 4A , JEV NS4A

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus NS4A antibody used in Western Blot (WB). GTX132028

GTX132028 WB Image

NS4A (JEV) antibody detects NS4A (JEV) protein by western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with NS4A (JEV) antibody (GTX132028) at a dilution of 1:1000.

REFERENCE

Application Reference

Anshu Agarwal et al. Microbiol Spectr 2022; 10 (3) : e0083022 Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells.
Application : WB, ICC/IF
Xiaochun Ma et al. Viruses 2022; 14 (4) Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection.
Keisuke Tabata et al. J Virol 2021; 95 (15) : e0223420 Endoplasmic Reticulum-Associated Degradation Controls Virus Protein Homeostasis, Which Is Required for Flavivirus Propagation.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169