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Japanese encephalitis virus NS4B antibody

Anti-Japanese encephalitis virus NS4B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125865
Anti-Japanese encephalitis virus NS4B antibody used in Western Blot (WB). GTX125865
Anti-Japanese encephalitis virus NS4B antibody used in Western Blot (WB). GTX125865

Cat. No. GTX125865

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus NS4B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Japanese encephalitis virus infect BHK-21

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Full length Japanese encephalitis virus JEV NS4b Recombinant protein (Japanese Encephalitis Virus strain Jaoars982)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173957

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Non-structural protein 4B , Japanese encephalitis Virus NS4B , JEV Non-structural protein 4B , JEV NS4B

Cellular Localization

Protein C: Virion (Potential) , Host membrane; Single-pass membrane protein , Small envelope protein M: Virion (Potential) , Envelope protein E: Virion (Potential) , Host membrane; Multi-pass membrane protein , Non-structural protein 4A: Host membrane; Si

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus NS4B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125865

GTX125865 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Infected BHK-21 cells , using non-structural protein NS4b (Japanese encephalitis virus)(GTX125865) antibody at 1:2000 dilution.

Anti-Japanese encephalitis virus NS4B antibody used in Western Blot (WB). GTX125865

GTX125865 WB Image

Sample (20 ug of whole cell lysate)
A: BHK-21  B: Dengue virus 2 infect BHK-21 C: Japanese encephalitis virus infect BHK-21 12% SDS PAGE
GTX125865 diluted at 1:1000

Anti-Japanese encephalitis virus NS4B antibody used in Western Blot (WB). GTX125865

GTX125865 WB Image

The data was published in the journal Viruses in 2020. PMID: 32075019

REFERENCE

Application Reference

Xiaochun Ma et al. Viruses 2022; 14 (4) Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection.
Keisuke Tabata et al. J Virol 2021; 95 (15) : e0223420 Endoplasmic Reticulum-Associated Degradation Controls Virus Protein Homeostasis, Which Is Required for Flavivirus Propagation.
Application : WB
Kaufusi PH et al. Viruses 2020; 12 (2) Selective Reactivity of Anti-Japanese Encephalitis Virus NS4B Antibody Towards Different Flaviviruses.
Application : WB
Lu CY et al. Viruses 2017; 9 (4) Single-Round Infectious Particle Antiviral Screening Assays for the Japanese Encephalitis Virus.
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169