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JunB antibody

Orthogonal
Anti-JunB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79258
Anti-JunB antibody used in ChIP assay (ChIP assay). GTX79258

Cat. No. GTX79258

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
200 μg ($399)
See all JunB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 10 μg/ml
ICC/IF 2μg/ml
FACS 3-5 μg/10⁶ cells
IP Assay dependent
ChIP assay 1-3 μl
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues D(45) P Y R G L K G P G A R G P G P E(61) of mouse JunB.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_11177451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

jun B proto-oncogene

Cellular Localization

Nucleus

Background

Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

Database

Research Area

DATA IMAGES

Anti-JunB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79258

GTX79258 ICC/IF Image

ICC/IF analysis of PC-3 cells using GTX79258 JunB antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-JunB antibody used in ChIP assay (ChIP assay). GTX79258

GTX79258 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX79258 JunB antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

REFERENCE

Application Reference

Tai F et al. Nat Commun 2020; 11 (1) : 258 Enhanced JunD/RSK3 signalling due to loss of BRD4/FOXD3/miR-548d-3p axis determines BET inhibition resistance.
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REVIEW

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Package List Price ($)
$ 399