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JunB (phospho Ser259) antibody

Anti-JunB (phospho Ser259) antibody used in Western Blot (WB). GTX50106
Anti-JunB (phospho Ser259) antibody used in IHC (Paraffin sections) (IHC-P). GTX50106

Cat No. GTX50106

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from human JunB.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

JunB proto-oncogene, AP-1 transcription factor subunit , AP-1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-JunB (phospho Ser259) antibody used in Western Blot (WB). GTX50106

GTX50106 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with EGF using GTX50106 JunB (phospho Ser259) antibody.

Anti-JunB (phospho Ser259) antibody used in IHC (Paraffin sections) (IHC-P). GTX50106

GTX50106 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50106 JunB (phospho Ser259) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf