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JunB (phospho Ser259) antibody

Orthogonal
Anti-JunB (phospho Ser259) antibody used in IHC (Paraffin sections) (IHC-P). GTX50106
Anti-JunB (phospho Ser259) antibody used in Western Blot (WB). GTX50106

Cat. No. GTX50106

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all JunB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from human JunB.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

JunB proto-oncogene, AP-1 transcription factor subunit , AP-1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-JunB (phospho Ser259) antibody used in IHC (Paraffin sections) (IHC-P). GTX50106

GTX50106 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50106 JunB (phospho Ser259) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-JunB (phospho Ser259) antibody used in Western Blot (WB). GTX50106

GTX50106 WB Image

Orthogonal

WB analysis of extracts from HT-29 cells untreated or treated with EGF using GTX50106 JunB (phospho Ser259) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399