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JunD antibody

Orthogonal
Anti-JunD antibody used in ChIP assay (ChIP assay). GTX79259
Anti-JunD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79259

Cat. No. GTX79259

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
200 μg ($399)
See all JunD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 10 μg/ml
ICC/IF 2-3 μg/ml
FACS 3-5 μg/10⁶ cells
ChIP assay 1-3 μl
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues G(329) C Q L L P Q H Q V P A Y(341) of mouse JunD.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11173549

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

jun D proto-oncogene , Jund1

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES

Anti-JunD antibody used in ChIP assay (ChIP assay). GTX79259

GTX79259 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX79259 JunD antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

Anti-JunD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79259

GTX79259 ICC/IF Image

ICC/IF analysis of PC-3 cells using GTX79259 JunD antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399