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JunD (phospho Ser255) antibody

Orthogonal
Anti-JunD (phospho Ser255) antibody used in IHC (Paraffin sections) (IHC-P). GTX50107
Anti-JunD (phospho Ser255) antibody used in Western Blot (WB). GTX50107

Cat. No. GTX50107

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all JunD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from human JunD.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

JunD proto-oncogene, AP-1 transcription factor subunit , AP-1

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216). [provided by RefSeq, Nov 2013]

Database

Research Area

DATA IMAGES

Anti-JunD (phospho Ser255) antibody used in IHC (Paraffin sections) (IHC-P). GTX50107

GTX50107 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50107 JunD (phospho Ser255) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-JunD (phospho Ser255) antibody used in Western Blot (WB). GTX50107

GTX50107 WB Image

Orthogonal

WB analysis of extracts from 293 cells using GTX50107 JunD (phospho Ser255) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399