20 mM HEPES (pH 7.9), 100 mM KCl, 1 mM MgCl₂ , 20% glycerol, 0.5 mM PMSF, and 0.5 mM DTT
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
4 mg/ml (Please refer to the vial label for the specific concentration.)
The nuclear extract was prepared from the heat shock-treated Jurkat human T cell leukemia cell line (clone E6-1). Cells were cultured in medium heated at 458C for 45 minutes immediately prior to harvesting.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.