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Human Jurkat, Clone E6-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The Jurkat cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Jurkat cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.
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