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Jurkat (human [E6-1] T lymphocyte; acute T cell leukemia) cell line slides

Cat No. GTX25533

Application

 ICC/IF

Species

 Human
Package
5 slide ($199)
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PRODUCT

Summary

Jurkat, Clone E6-1 (T lymphocyte; acute T cell leukemia)

Human Jurkat, Clone E6-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The Jurkat cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Jurkat cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.

Tissue type

Tumor

APPLICATION

Application Note

Jurkat cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.

PROPERTIES

Storage

Stable for up to 6 months at 4˚C under desiccate conditions.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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Package List Price ($)
$ 199