This antibody may cross react with human N-Ras and H-Ras.
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human KRAS (1-186aa) purified from E. coli
By protein-A affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Kras Proto-Oncogene, Gtpase,C-K-Ras,Cfc2,K-Ras2A,K-Ras2B,K-Ras4A,K-Ras4B,K-Ras,Ki-Ras,Kras1,Kras2,Ns,Ns3,Rald,Rask2,C-Ki-Ras2,Kras
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
GTX31957 ICC/IF Image
ICC/IF analysis of KRAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
GTX31957 WB Image
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : HepG2 cell lysate
Lane 3. : Ramos cell lysate
Lane 4. : A549 cell lysate
Lane 5. : Balb/3T3 cell lysate
GTX31957 FACS Image
Flow cytometry analysis of KRAS in A549 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).