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K-Ras antibody [AT2F8]

Anti-K-Ras antibody [AT2F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31957
Anti-K-Ras antibody [AT2F8] used in Flow cytometry (FACS). GTX31957
Anti-K-Ras antibody [AT2F8] used in Western Blot (WB). GTX31957

Cat. No. GTX31957

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2F8

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all K-Ras products

APPLICATION

Application Note

WB: 1:500. ICC/IF: 1:100

Calculated MW

22 kDa. ( Note )

Product Note

This antibody may cross react with human N-Ras and H-Ras.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human KRAS (1-186aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2

Cellular Localization

Cell membrane , Cytoplasm, cytosol

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-K-Ras antibody [AT2F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31957

GTX31957 ICC/IF Image

ICC/IF analysis of KRAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Anti-K-Ras antibody [AT2F8] used in Flow cytometry (FACS). GTX31957

GTX31957 FACS Image

Flow cytometry analysis of KRAS in A549 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Anti-K-Ras antibody [AT2F8] used in Western Blot (WB). GTX31957

GTX31957 WB Image

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399