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K-Ras antibody

Anti-K-Ras antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32694
Anti-K-Ras antibody used in Western Blot (WB). GTX32694
Anti-K-Ras antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32694

Cat. No. GTX32694

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all K-Ras products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

This antibody may cross react with N-Ras and H-Ras proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human KRAS

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2

Cellular Localization

Cell membrane,Cytoplasm, cytosol

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-K-Ras antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32694

GTX32694 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32694 K-Ras antibody.
Blue : DAPI
Dilution : 1:100

Anti-K-Ras antibody used in Western Blot (WB). GTX32694

GTX32694 WB Image

WB analysis of various sample lysates using GTX32694 K-Ras antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-K-Ras antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32694

GTX32694 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32694 K-Ras antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399