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KAP1 antibody [20C1]

Orthogonal
Anti-KAP1 antibody [20C1] used in Western Blot (WB). GTX80695
Anti-KAP1 antibody [20C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80695
Anti-KAP1 antibody [20C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80695
Anti-KAP1 antibody [20C1] used in Western Blot (WB). GTX80695

Cat. No. GTX80695

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 20C1

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP assay, Gel supershift assays

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all KAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
ICC/IF 2 μg/ml
IP Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [20C1] used in Western Blot (WB). GTX80695

GTX80695 WB Image

WB analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].

Anti-KAP1 antibody [20C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80695

GTX80695 ICC/IF Image

ICC/IF analysis of gamma irradiated HeLa cells using GTX80695 KAP1 antibody [20C1].

Anti-KAP1 antibody [20C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80695

GTX80695 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80695 KAP1 antibody [20C1]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-KAP1 antibody [20C1] used in Western Blot (WB). GTX80695

GTX80695 WB Image

Orthogonal

WB analysis of nuclear enriched extracts (30 μg lysate) of HEK 293 treated with Hydrogen peroxide (100um Hydrogen peroxide for 10 min) (Lane 1), HEK 293 (Lane 2), A549 (Lane 3), HEL 92.1.7 (Lane 4), PC-3 (Lane 5), and Caco-2 (Lane 6) using GTX80695 KAP1 antibody [20C1].
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399