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KAP1 antibody [4E1-D12-F8]

Anti-KAP1 antibody [4E1-D12-F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49179
Anti-KAP1 antibody [4E1-D12-F8] used in Western Blot (WB). GTX49179
Anti-KAP1 antibody [4E1-D12-F8] used in IHC (Paraffin sections) (IHC-P). GTX49179

Cat No. GTX49179

Host

Mouse

Clonality

Monoclonal

Clone Name

4E1-D12-F8

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human KAP1 protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tripartite Motif Containing 28 , Kap1 , Ppp1R157 , Rnf96 , Tf1B , Tif1B , Trim28

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [4E1-D12-F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49179

GTX49179 ICC/IF Image

ICC/IF analysis of HeLa cells using KAP1 antibody at a dilution of 1:200.

Anti-KAP1 antibody [4E1-D12-F8] used in Western Blot (WB). GTX49179

GTX49179 WB Image

WB analysis of 293T and HepG2 lysates using KAP1 antibody at a dilution of 1:1,000.

Anti-KAP1 antibody [4E1-D12-F8] used in IHC (Paraffin sections) (IHC-P). GTX49179

GTX49179 IHC-P Image

IHC-P analysis of human spleen tissue using KAP1 antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf