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KAP1 antibody [4E1-D12-F8]

Anti-KAP1 antibody [4E1-D12-F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49179
Anti-KAP1 antibody [4E1-D12-F8] used in Western Blot (WB). GTX49179
Anti-KAP1 antibody [4E1-D12-F8] used in IHC (Paraffin sections) (IHC-P). GTX49179

Cat. No. GTX49179

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E1-D12-F8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all KAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7.4), 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human KAP1 protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

RRID

AB_2887760

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [4E1-D12-F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49179

GTX49179 ICC/IF Image

ICC/IF analysis of HeLa cells using KAP1 antibody at a dilution of 1:200.

Anti-KAP1 antibody [4E1-D12-F8] used in Western Blot (WB). GTX49179

GTX49179 WB Image

WB analysis of 293T and HepG2 lysates using KAP1 antibody at a dilution of 1:1,000.

Anti-KAP1 antibody [4E1-D12-F8] used in IHC (Paraffin sections) (IHC-P). GTX49179

GTX49179 IHC-P Image

IHC-P analysis of human spleen tissue using KAP1 antibody.

REFERENCE

Application Reference

Qingmei Meng et al. Redox Rep 2021; 26 (1) : 160-169 Possible relationship between mitochondrial changes and oxidative stress under low dose-rate irradiation.
Application : WB
Reactivity : Human
Sarkar A et al. Mol Cell Biochem 2020; Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies.
Application : WB
Qi F et al. J Radiat Res 2020; 61 (3) : 368-375 FXR1 is a novel MRE11-binding partner and participates in oxidative stress responses.
Application : WB
Reactivity : Human
Sarkar A et al. Haematologica 2020; (Epub) Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399