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KAP1 antibody, C-term

Anti-KAP1 antibody, C-term used in Flow cytometry (FACS). GTX89879
Anti-KAP1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89879
Anti-KAP1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89879
Anti-KAP1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89879

Cat No. GTX89879

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P, FACS, ChIP assay

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 10μg/ml
IHC-P 2-4μg/ml
FACS Assay dependent
ChIP assay Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the nucleus of HeLa and U2OS cells.

IHC-P
Human Breast shows nuclear staining in epithelial duct cells.

Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89879-PEP).

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody, C-term used in Flow cytometry (FACS). GTX89879

GTX89879 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX89879 KAP1 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-KAP1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89879

GTX89879 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX89879 KAP1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-KAP1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89879

GTX89879 IHC-P Image

IHC-P analysis of human breast using GTX89879 KAP1 antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

Anti-KAP1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89879

GTX89879 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX89879 KAP1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf