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KAP1 antibody [GT1161]

Recombinant antibody
Anti-KAP1 antibody [GT1161] used in Western Blot (WB). GTX08968
Anti-KAP1 antibody [GT1161] used in Western Blot (WB). GTX08968
Anti-KAP1 antibody [GT1161] used in IHC (Paraffin sections) (IHC-P). GTX08968
Anti-KAP1 antibody [GT1161] used in IHC (Paraffin sections) (IHC-P). GTX08968
Anti-KAP1 antibody [GT1161] used in IHC (Paraffin sections) (IHC-P). GTX08968
Anti-KAP1 antibody [GT1161] used in Immunoprecipitation (IP). GTX08968

Cat. No. GTX08968

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1161

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all KAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan KAP1

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [GT1161] used in Western Blot (WB). GTX08968

GTX08968 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [GT1161] (GTX08968) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KAP1 antibody [GT1161] used in Western Blot (WB). GTX08968

GTX08968 WB Image

WB analysis of various samples using GTX08968 KAP1 antibody [GT1161].
Dilution : 1:1000
Loading : 25 μg

Anti-KAP1 antibody [GT1161] used in IHC (Paraffin sections) (IHC-P). GTX08968

GTX08968 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX08968 KAP1 antibody [GT1161].
Dlution : 1:100

Anti-KAP1 antibody [GT1161] used in IHC (Paraffin sections) (IHC-P). GTX08968

GTX08968 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX08968 KAP1 antibody [GT1161].
Dlution : 1:100

Anti-KAP1 antibody [GT1161] used in IHC (Paraffin sections) (IHC-P). GTX08968

GTX08968 IHC-P Image

IHC-P analysis of hmuan appendix tissue section using GTX08968 KAP1 antibody [GT1161].
Dlution : 1:100

Anti-KAP1 antibody [GT1161] used in Immunoprecipitation (IP). GTX08968

GTX08968 IP Image

IP analysis of HeLa cell lysate using GTX08968 KAP1 antibody [GT1161].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399