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KAP1 antibody [HL1539]

Recombinant antibody
Anti-KAP1 antibody [HL1539] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637013
Anti-KAP1 antibody [HL1539] used in Western Blot (WB). GTX637013
Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013
Anti-KAP1 antibody [HL1539] used in Western Blot (WB). GTX637013
Anti-KAP1 antibody [HL1539] used in Western Blot (WB). GTX637013
Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013
Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013
Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013

Cat. No. GTX637013

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1539

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [HL1539] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637013

GTX637013 ICC/IF Image

KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-KAP1 antibody [HL1539] used in Western Blot (WB). GTX637013

GTX637013 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (GTX637013) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013

GTX637013 IHC-P Image

KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KAP1 antibody [HL1539] used in Western Blot (WB). GTX637013

GTX637013 WB Image

Human tissue extract (30 μg) was separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (GTX637013) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KAP1 antibody [HL1539] used in Western Blot (WB). GTX637013

GTX637013 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (GTX637013) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013

GTX637013 IHC-P Image

KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013

GTX637013 IHC-P Image

KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KAP1 antibody [HL1539] used in IHC (Paraffin sections) (IHC-P). GTX637013

GTX637013 IHC-P Image

KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KAP1 stained by KAP1 antibody [HL1539] (GTX637013) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations