GeneTex
United States (US)

KAP1 antibody [N1N2], N-term

GTX102227_39757_IFA_19011506_413.jpg
GTX102227_WB_M_19011506_139.jpg
GTX102227_39757_WB_M_brain_19011506_713.jpg
GTX102227_39757_20141120_IHC_M_2_19011506_601.jpg
GTX102227_39757_20141120_IHC_M_19011506_114.jpg
GTX102227_WB_19011506_551.jpg

Cat No. GTX102227

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , NIH-3T3 , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripartite Motif Containing 28,Kap1,Ppp1R157,Rnf96,Tf1B,Tif1B,Trim28

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102227_39757_IFA_19011506_413.jpg

GTX102227 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KAP1(GTX102227) antibody at 1:200 dilution.

GTX102227_WB_M_19011506_139.jpg

GTX102227 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX102227 diluted at 1:1000

GTX102227_39757_WB_M_brain_19011506_713.jpg

GTX102227 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102227 diluted at 1:1000

GTX102227_39757_20141120_IHC_M_2_19011506_601.jpg

GTX102227 IHC-P Image

KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
KAP1 antibody [N1N2], N-term (GTX102227) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102227_39757_20141120_IHC_M_19011506_114.jpg

GTX102227 IHC-P Image

KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
KAP1 antibody [N1N2], N-term (GTX102227) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102227_WB_19011506_551.jpg

GTX102227 WB Image

Sample (30 ug of whole cell lysate)

A: Hela

B: Hep G2 (GTX27900)

7.5% SDS PAGE

GTX102227 diluted at 1:10000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169