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KAP1 antibody [N1N2], N-term

Anti-KAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

Cat No. GTX102227

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , NIH-3T3 , mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037323

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102227

GTX102227 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KAP1(GTX102227) antibody at 1:200 dilution.

Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX102227 diluted at 1:1000

Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102227 diluted at 1:1000

Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227

GTX102227 IHC-P Image

KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
KAP1 antibody [N1N2], N-term (GTX102227) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227

GTX102227 IHC-P Image

KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
KAP1 antibody [N1N2], N-term (GTX102227) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Sample (30 ug of whole cell lysate)

A: Hela

B: Hep G2 (GTX27900)

7.5% SDS PAGE

GTX102227 diluted at 1:10000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf