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KAP1 antibody [N3C2], Internal

Orthogonal
Anti-KAP1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in ChIP assay (ChIP assay). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in Western Blot (WB). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in Western Blot (WB). GTX102226
Anti-KAP1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102226

Cat. No. GTX102226

Reference 12
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all KAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037324

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102226

GTX102226 IP Image

KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of KAP1 protein by 2 μg of KAP1 antibody (GTX102226) 7.5% SDS-PAGE The immunoprecipitated KAP1 protein was detected by KAP1 antibody (GTX102226) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-KAP1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102226

GTX102226 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1(GTX102226) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KAP1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102226

GTX102226 IHC-P Image

KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KAP1 antibody [N3C2], Internal used in ChIP assay (ChIP assay). GTX102226

GTX102226 ChIP assay Image

ChIP assay followed by QPCR on a known KAP1-binding region
within the ZNF180 3’UTR (Mol Cell Biol, 2011, Lyengar et al)

Anti-KAP1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102226

GTX102226 IHC-P Image

KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KAP1 antibody [N3C2], Internal used in Western Blot (WB). GTX102226

GTX102226 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX102226 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KAP1 antibody [N3C2], Internal used in Western Blot (WB). GTX102226

GTX102226 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KAP1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102226

GTX102226 ICC/IF Image

KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KAP1 stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

REFERENCE

Application Reference

Faith C Fowler et al. Elife 2022; 11 DNA-PK promotes DNA end resection at DNA double strand breaks in G0 cells.
Application : WB
Reactivity : Mouse
Bo-Ruei Chen et al. Elife 2021; 10 LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells.
Application : WB
Reactivity : Mouse
Chen BR et al. DNA Repair (Amst) 2021; 108 : 103217 The RNF8 and RNF168 Ubiquitin Ligases Regulate Pro- and Anti-Resection Activities at Broken DNA Ends During Non-Homologous End Joining.
Application : WB
Reactivity : Mouse
Liu B et al. Pathol Oncol Res 2019; 25 (4) : 1645-1652 Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus.
Hsieh YH et al. Nutrients 2018; 10 (9) Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice.
Show More +
Submit a Reference

REVIEW

KAP1 antibody [N3C2], Internal Cat. No. GTX102226

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-KAP1 antibody [N3C2], Internal used in Western Blot (WB). GTX102226
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2018.PMID: 30200495
Submit a Review
Application Reference
Faith C Fowler et al. Elife 2022; 11 DNA-PK promotes DNA end resection at DNA double strand breaks in G0 cells.
Application : WB
Reactivity : Mouse
Bo-Ruei Chen et al. Elife 2021; 10 LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells.
Application : WB
Reactivity : Mouse
Chen BR et al. DNA Repair (Amst) 2021; 108:103217 The RNF8 and RNF168 Ubiquitin Ligases Regulate Pro- and Anti-Resection Activities at Broken DNA Ends During Non-Homologous End Joining.
Application : WB
Reactivity : Mouse
Liu B et al. Pathol Oncol Res 2019; 25 (4):1645-1652 Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus.
Huang TH et al. Cell Cycle 2018; 17 (12):1413-1424 The histone chaperone ASF1 regulates the activation of ATM and DNA-PKcs in response to DNA double-strand breaks.
Hsieh YH et al. Nutrients 2018; 10 (9) Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice.
Hung PJ et al. Mol Cell 2018; 71 (2):332-342.e8 MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining.
Application : WB
Reactivity : Mouse
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Wang L et al. J Cell Biochem 2016; 117 (6):1429-38 BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis.
Application : ICC/IF
Reactivity : Human
Aslanian A et al. DNA Repair (Amst) 2014; 15 (Epub):29-38 Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.
Application : WB
Reactivity : Human
Tubbs AT et al. Mol Cell Biol 2014; KAP-1 Promotes Resection of Broken DNA Ends Not Protected by γ-H2AX and 53BP1 in G1-Phase Lymphocytes.
Application : WB
Reactivity : Mouse
Lee BS et al. Mol Cell Biol 2013; 33 (18):3568-79 Functional intersection of ATM and DNA-dependent protein kinase catalytic subunit in coding end joining during V(D)J recombination.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169