GeneTex
United States (US)

KAP1 antibody [N3C2], Internal

GTX102226_39911_IP_19011506_930.jpg
GTX102226_39911_20150410_IFA_19011506_112.jpg
GTX102226_WB_19011506_813.jpg
GTX102226_CHIP_19011506_901.jpg
GTX102226_WB_M_19011506_553.jpg
GTX102226_39911_IHC_19011506_667.jpg
GTX102226_39911_IFA_19011506_597.jpg

Cat No. GTX102226

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

H1299 , HeLa , NIH-3T3

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripartite Motif Containing 28,Kap1,Ppp1R157,Rnf96,Tf1B,Tif1B,Trim28

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102226_39911_IP_19011506_930.jpg

GTX102226 IP Image

KAP1 antibody immunoprecipitates KAP1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of KAP1 protein by 2 μg of KAP1 antibody (GTX102226) 7.5% SDS-PAGE The immunoprecipitated KAP1 protein was detected by KAP1 antibody (GTX102226) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102226_39911_20150410_IFA_19011506_112.jpg

GTX102226 ICC/IF Image

KAP1 antibody [N3C2], Internal detects KAP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KAP1 protein stained by KAP1 antibody [N3C2], Internal (GTX102226) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.

GTX102226_WB_19011506_813.jpg

GTX102226 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
B: HeLa
7.5% SDS PAGE
GTX102226 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102226_CHIP_19011506_901.jpg

GTX102226 ChIP assay Image

ChIP assay followed by QPCR on a known KAP1-binding region
within the ZNF180 3’UTR (Mol Cell Biol, 2011, Lyengar et al)

GTX102226_WB_M_19011506_553.jpg

GTX102226 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX102226 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102226_39911_IHC_19011506_667.jpg

GTX102226 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using KAP1(GTX102226) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102226_39911_IFA_19011506_597.jpg

GTX102226 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using KAP1(GTX102226) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169