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KAP1 antibody

Anti-KAP1 antibody used in Western Blot (WB). GTX64422
Anti-KAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64422
Anti-KAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64422
Anti-KAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64422
Anti-KAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64422

Cat No. GTX64422

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:3000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human TRIM28 (NP_005753.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody used in Western Blot (WB). GTX64422

GTX64422 WB Image

WB analysis of various sample lysates using GTX64422 KAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-KAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64422

GTX64422 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64422 KAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-KAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64422

GTX64422 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64422 KAP1 antibody.
Dilution : 1:100

Anti-KAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64422

GTX64422 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64422 KAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-KAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64422

GTX64422 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64422 KAP1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf