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KCC2 antibody [HL2535]

Recombinant antibody Orthogonal
Anti-KCC2 antibody [HL2535] used in Western Blot (WB). GTX638904
Anti-KCC2 antibody [HL2535] used in IHC (Paraffin sections) (IHC-P). GTX638904
Anti-KCC2 antibody [HL2535] used in IHC (Paraffin sections) (IHC-P). GTX638904
Anti-KCC2 antibody [HL2535] used in Western Blot (WB). GTX638904
Anti-KCC2 antibody [HL2535] used in Western Blot (WB). GTX638904
Anti-KCC2 antibody [HL2535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638904

Cat. No. GTX638904

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2535

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KCC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Product Note

This antibody was raised against mouse KCC2 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of mouse KCC2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 12, member 5 , KCC2 , mKIAA1176

Cellular Localization

Cell projection, dendrite,Cell membrane

Database

Research Area

DATA IMAGES

Anti-KCC2 antibody [HL2535] used in Western Blot (WB). GTX638904

GTX638904 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (GTX638904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-KCC2 antibody [HL2535] used in IHC (Paraffin sections) (IHC-P). GTX638904

GTX638904 IHC-P Image

KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
KCC2 stained by KCC2 antibody [HL2535] (GTX638904) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KCC2 antibody [HL2535] used in IHC (Paraffin sections) (IHC-P). GTX638904

GTX638904 IHC-P Image

KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
KCC2 stained by KCC2 antibody [HL2535] (GTX638904) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KCC2 antibody [HL2535] used in Western Blot (WB). GTX638904

GTX638904 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (GTX638904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-KCC2 antibody [HL2535] used in Western Blot (WB). GTX638904

GTX638904 WB Image

Orthogonal

Unboiled and boiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (GTX638904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KCC2 antibody [HL2535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638904

GTX638904 ICC/IF Image

KCC2 antibody [HL2535] detects KCC2 protein at Glogi by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KCC2 stained by KCC2 antibody [HL2535] (GTX638904) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations