KCC2 antibody [HL2535]

KCC2 antibody [HL2535] detects KCC2 protein at Glogi by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KCC2 stained by KCC2 antibody [HL2535] (GTX638904) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (GTX638904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
KCC2 stained by KCC2 antibody [HL2535] (GTX638904) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

KCC2 antibody [HL2535] detects KCC2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
KCC2 stained by KCC2 antibody [HL2535] (GTX638904) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Unboiled and boiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (GTX638904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [HL2535] (GTX638904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.